Plant Metallothioneins: Putative Functions Identified by Promoter Analysis in Silico
Identifieur interne : 001388 ( Main/Exploration ); précédent : 001387; suivant : 001389Plant Metallothioneins: Putative Functions Identified by Promoter Analysis in Silico
Auteurs : Gra Yna D Browska [Pologne]Source :
- Acta Biologica Cracoviensia Series Botanica [ 0001-5296 ] ; 2012.
English descriptors
- Teeft :
- Abiotic, Abre, Abre motif, Abscisic, Abscisic acid, Acta biologica cracoviensia series botanica, Amino acid sequences, Arabidopsis, Arabidopsis thaliana, Atmt, Brassica napus, Cdna, Circadian, Circadian clock, Cysteine, Cysteine residues, Database, Elaeis guineensis, Encoding, Esculentum, Ethylene, Experimental botany, Expression analysis, Febs letters, Functional analysis, Gene, Gene expression, Gene promoters, Goldsbrough, Guineensis, Hassinen, Heavy metal ions, Heavy metals, Ipomoea batatas, Largest number, Lycopersicon esculentum, Metallothionein, Metallothionein genes, Metallothionein promoters, Metallothioneins, Motif, Nicotiana glutinosa, Omidvar, Oryza, Oryza sativa, Osmt, Oxidative, Oxidative stress, Pathogen attack, Physiology, Pisum sativum, Plant biology, Plant cell, Plant journal, Plant metallothioneins, Plant physiology, Plant response, Plant science, Populus alba, Promoter, Promoter region, Promoter regions, Promoter sequence, Promoter sequences, Prosopis juliflora, Putative, Putative functions, Quinn, Regulatory elements, Regulatory motifs, Sativa, Sativa sequences, Silico analysis, Thaliana, Transgenic, Usha.
Abstract
Metallothioneins are low-molecular-weight proteins capable of covalently binding heavy metal ions due to the presence of many cysteine residues in their sequences. We analyzed the predicted amino acid sequences of 19 metallothionein (7 from Arabidopsis thaliana and 12 from Oryza sativa) and their promoter sequences in silico in order to determine the potential regulatory cis-elements present in the promoters of metallothionein genes, from which it is possible to determine the putative functions of these genes. The PlantCARE and PLACE databases provided information about the putative regulatory elements in the metallothionein promoters. Metal response element sequences were found in the promoters of eleven O. sativa and two Arabidopsis metallothionein genes. Copper response elements were identified in both model plants, usually in many copies, particularly in O. sativa. Both the high cysteine content and the presence of metal response motifs in the promoters support the suggestion that metallothioneins play a key role in metal detoxification. The most common putative element in the analyzed promoters was CIRCADIAN, which was present in five A. thaliana and eight O. sativa sequences. The methyl jasmonate response sequence, root-specific expression element and drought response element were found only in O. sativa metallothioneins. Light and low temperature response elements, biotic and abiotic stress elements, an abscisic acid-responsive element and an ethylene-responsive element occur in selected metallothionein promoters of both species. A few promoters have putative organ- and cell-specific regulatory elements. The presence of many different motifs in the promoters of the Arabidopsis and O. sativa genes implies that metallothioneins are general stress response proteins with many important functions in plants, including regulation of their normal development and adaptation to changing environmental conditions.
Url:
DOI: 10.2478/v10182-012-0031-x
Affiliations:
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We analyzed the predicted amino acid sequences of 19 metallothionein (7 from Arabidopsis thaliana and 12 from Oryza sativa) and their promoter sequences in silico in order to determine the potential regulatory cis-elements present in the promoters of metallothionein genes, from which it is possible to determine the putative functions of these genes. The PlantCARE and PLACE databases provided information about the putative regulatory elements in the metallothionein promoters. Metal response element sequences were found in the promoters of eleven O. sativa and two Arabidopsis metallothionein genes. Copper response elements were identified in both model plants, usually in many copies, particularly in O. sativa. Both the high cysteine content and the presence of metal response motifs in the promoters support the suggestion that metallothioneins play a key role in metal detoxification. The most common putative element in the analyzed promoters was CIRCADIAN, which was present in five A. thaliana and eight O. sativa sequences. The methyl jasmonate response sequence, root-specific expression element and drought response element were found only in O. sativa metallothioneins. Light and low temperature response elements, biotic and abiotic stress elements, an abscisic acid-responsive element and an ethylene-responsive element occur in selected metallothionein promoters of both species. A few promoters have putative organ- and cell-specific regulatory elements. The presence of many different motifs in the promoters of the Arabidopsis and O. sativa genes implies that metallothioneins are general stress response proteins with many important functions in plants, including regulation of their normal development and adaptation to changing environmental conditions.</div></front></TEI><affiliations><list><country><li>Pologne</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="D Browska, Gra Yna" sort="D Browska, Gra Yna" uniqKey="D Browska G" first="Gra Yna" last="D Browska">Gra Yna D Browska</name></noRegion><name sortKey="D Browska, Gra Yna" sort="D Browska, Gra Yna" uniqKey="D Browska G" first="Gra Yna" last="D Browska">Gra Yna D Browska</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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